In vitro Cultivation of Jatropha curcas L. cells for Growth Kinetic and Total Fatty acid Determination

The present study reports, In vitro cultivation of the Jatropha curcas (Linn.) plant cells for evaluation of growth kinetics and total fatty acid content. Plant cells were cultivated in three major synthetic media components in triplicates. Four growth measurement parameters were selected and the data was analyzed using analysis of variance followed by Tukey's honestly significant difference (P< 0.05). Among the parameters; the dry cell and fresh cell weight showed a significant variation between different media components than volumetric measurements. However, a consistent growth kinetics pattern was observed in dry cell weight of cells cultivated within individual medium. The biomass productivity was found to be 4.531, 2.265 and 1.903 gm l-1day-1 of cells cultivated in Murashige-Skoog, Woody Plant and Gamborg B5 media respectively. The maximum relative growth capacity of 0.621 and specific growth rate of 0.242 gm day-1 was found from cells cultivated in Murashige-Skoog media. The total fatty acid content obtained per dry cell weight was 5 % w/w using acidified petroleum ether extraction followed by transesterification. Thus, the dry cell weight method to determine growth kinetics and total fatty acid content in a liquid system is reliable and reproducible for further bioprocess and biotechnological studies of Jatropha.